《植物生理学报》 2009, 45(10): 977-980

几种生理因素对子房未授粉南瓜胚形成的影响

孙守如*, 翟庆慧, 胡建斌, 陈解放, 章鹏

河南农业大学园艺学院, 郑州450002

通信作者：孙守如；E-mail: ssr365@sina.com；Tel: 0371-63558096

摘　要：

以南瓜品种‘蜜本’为试材, 研究生理因素影响未授粉南瓜胚形成的结果表明: 开花前1 d的子房最适宜于胚的诱导;胚诱导前以35 ℃高温处理6 d后胚形成能力显著提高; 高浓度2,4-D促进胚的发生, 抑制愈伤组织的形成; 加有4.0 mg·L-12,4-D、0.5 mg·L-1 NAA和0.5 mg·L-1 6-BA的MS培养基上的出胚率最高(20.6±3.8)%, 每个子房块的平均出胚数达11.3±3.2。

关键词：南瓜; 未受精子房; 胚形成

收稿：2009-08-20   修定：2009-09-24

资助：河南省科技攻关项目(0624100004)和河南省重点科技攻关项目(9210211016)。

Effects of Several Physiological Factors on Embryo Formation in Unpollinated Ovary Culture of Pumpkin (Cucurbita moschata Duch. ex Poiret)

SUN Shou-Ru*, ZHAI Qing-Hui, HU Jian-Bin, CHEN Jie-Fang, ZHANG Peng

College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China

Corresponding author: SUN Shou-Ru; E-mail: ssr365@sina.com; Tel: 0371-63558096

Abstract:

Effects of several physiological factors on embryo formation in unpollinated pumpkin ovary culture were studied using ‘Miben’ pumpkin (Cucurbita moschata) as experimental material. The results showed that the ovaries at the stage of 1 d before anthesis were optimal for embryo induction. Heat treatment of 35℃ for 6 d before embryo induction remarkably enhanced the potential of embryo formation in unpollinated ovary. High concentration of 2,4-D promoted embryo formation in unpollinated ovary and inhibited callus formation. The ovary explants cultured in MS medium containing 4.0 mg·L^-1 2,4-D, 0.5 mg·L^-1 NAA and 0.5 mg·L^-1 6-BA gave highest embryo formation rate [(20.6±3.8)%] with the mean embryo number of 11.3±3.2 per ovary segment.

Key words: pumpkin; unpollinated ovary; embryo formation